MRSA detection based on up-conversion glassfibres
IGF 20304 N
The aim of the research project is to develop an optochemical sensor that enables MRSA to be detected directly on site. The optochemical sensor contains an upconversion glass fiber, i.e. phage-functionalized upconversion particles (UCPs) are bound on the surface of the glass fiber and serve as sensors. When NIR light is passed through the upconversion fiber, the UCPs are excited to luminesce, the luminescent light is coupled into the fiber and detected at the photodetector.
The detection principle of the optochemical sensor is based on the quenching of the luminescence of phage-functionalized UCPs after binding of living S. aureus cells and labeling of bound MRSA cells by antibody-functionalized gold nanoparticles, which reduces the intensity of the luminescent light.
The optochemical sensor allows immediate identification and isolation of MRSA-colonized patients. Using the sensor, cleaning and hygiene service providers of the Hygiene Commission of Hospitals can immediately demonstrate the success of cleaning/disinfection procedures performed. If necessary, corrective measures can be initiated immediately.
Critical points that are subject to frequent skin/hand contact can be determined by the Hygiene Commission and regularly examined by cleaning and hygiene service providers for MRSA contamination. With the help of the data obtained, hygiene specialists can continuously optimise hygiene plans and improve strategies for avoiding contaminations, which are of essential importance for the prevention of nosocomial MRSA cases and the containment of the spread of MRSA, with the cooperation of external service providers.